Saturday, May 14, 2005
GAs used to study protozoan glyoxalase pathway
The glyoxalase pathway of Leishmania infantum was kinetically characterized as a trypanothione-dependent system. Using time course analysis based on parameter fitting with a genetic algorithm, kinetic parameters were estimated for both enzymes, with trypanothione derived substrates. A Km of 0.253 mM and a V of 0.21 µmol·min–1·mg–1for glyoxalase I, and a Km of 0.098 mM and a V of 0.18 µmol·min–1·mg–1 for glyoxalase II, were obtained.
The research was a collaboration between Portugese researchers at the University of Porto and the University of Lisbon.